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ID 18189
Eprint ID
18189
FullText URL
Thumnail 80_1167.pdf 5.73 MB
Title Alternative
Studies on Tissue Culture of Spleen Chapter Ⅲ Vital Observation of Splenic Reticulum Cells by Fluorochrominized Tissue Culture
Author
Kibata, Masayoshi
Abstract
1) Fluorochrominized tissue culture of spleen was established by the similar method used for bone marrow culture devised in our clinic. And vital observation of splenic reticulum cells was done by fluorochrominized tissue culture method by the use of rectangular cover slip method which has been mentioned in the chapter I. 2) Each type of reticulum cells showed characteristic fluorochrominized findings when stained with acridine orange. Granules and vaculoles stained with acridine orange showed nearly similar findings to neutral red granules and vacuoles as described in the chapter Ⅱ. 3) By this method, the existence of transitional forms between each type of reticulum cells and maturation process were confirmed in the series of types Ⅰ, Ⅱ and Ⅲ reticulum cells, as have been already described by other methods in the chapter Ⅰ and Ⅱ. 4) Part of acridine orange stainable vacuoles were thought to be secondary to toxic effects of acridine orange stain. 5) Changes of fluorochrominized granules and vacuoles were observed successively in the degenaration of type Ⅲ reticulum cells.
Published Date
1968-12-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume80
Issue
issue11-12
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
1167
End Page
1180
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/80/11-12/80_11-12_1167/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma