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ID 18125
Eprint ID
18125
FullText URL
Thumnail 80_631.pdf 708 KB
Title Alternative
Studies on the Measurement of Human Blood Catalase Activity and the Screening Method of Hypocatalasemia by Spectrophotometry Part 4. A simple method of measuring human blood catalase activity and the screening test for hypocatalasemia
Author
Nakagawa, Yoshito
Abstract
It is generally accepted that modified Euler-Josephson's method of titration gives the value of catalase activity, “Kcat” as the standard value. Whereas by the author's method taking the value of catalase activity as "k" measured, for example, at room temperature, recorded on an autorecording apparatus, it will be seen that we get the formula, Kcat=1/1.035 (100/90K+0.513) The present study has led to the following conclusions: This method is characterized by the fact that it is useful for the screening test for hypocatalasemia as well as for simultaneous measurement of the catalase activity in human blood. It is suggested that the level of the screening be fixed more appropriately at 2.4. Attempts have been made to use a portable UV-colorimeter to the screening but it seemed that this apparatus is not suitable for the purpose. This method can measure not only the activity of purified catalase but also that of a partially purified catalase. It can also be applied for the measurement of catalase activity in tissues other than blood. It is anticipated that this method can also be useful for the investigation of hypocatalasemic blood catalase from the aspect of enzyme kinetics.
Published Date
1968-06-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume80
Issue
issue5-6
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
631
End Page
635
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/80/5-6/80_5-6_631/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma