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ID 17603
Eprint ID
17603
FullText URL
Thumnail 88_397.pdf 183 KB
Title Alternative
Quantitative determination of urinary styrene metabolites by means of Gas chromatography
Author
Saeki, Takao
Abstract
Quantitative determination of urinary mandelic acid (MA) phenylglyoxylic acid (PGA) and hippuric acid (HA) (metabolites of styrene monomer.) was conducted. For the determination of urinary MA and PGA, a known amount of myristric acid (internal standard) is added to the urine, before its extraction with mixture of ethyl acetate and ethyl ether. After evaporation of the solvents, the acids are silylated with bis (trimethylsilyl) acetamide. For the determination of urinary HA, HA in ethyl acetate extract from urine, is methylated with diazomethane. Thereafter they were injected into gas chromatograph. Rats were administrated styrene intraperitoneally, and their urins were collected as time lapses. Maximum excretion of MA and PGA reached after 24 hours from styrene injection and that of HA did after 6 hours. Total excretion of MA was caluculated to be 45.9% of styrene administrated that of PGA 23.9%, that of 18.1% and that of all metabolites 87.9%, respectively. Discussion was made between amount of styrene exposed with people and amount of their urinary metabolites.
Published Date
1976-06-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume88
Issue
issue5-6
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
397
End Page
401
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/88/5-6/88_5-6_397/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma