このエントリーをはてなブックマークに追加
ID 17404
Eprint ID
17404
FullText URL
90_901.pdf 605 KB
Title Alternative
Spontaneous malignant transformation of rat liver cells in long-term culture Part 2. Acquirement of tumor-producing capacity and chromosomal alteration on the eleven epithelial liver cell lines of Donryu rats
Author
Nishizaki, Shizuo
Abstract
1) For past 10 years, 11 epithelial liver cell lines from baby rats of Donryu strain have been established in our laboratory. 2) All cultured cells in the 11 cell lines showed the same epithelial morphology in early culture passages irrespective of culture methods. 3) The cultured cells progressively deviated from normal to abnormal with time in chromosome as well as in morphology during long-term cultivation; normal diploid cells shifted to aneuploid and also homogeneous epithelial cells changed to heterogeneous in shape. 4) The cells in all of the 11 cell lines eventually underwent spontaneous malignant transformation in vitro after about 600 culture days and produced tumors in syngeneic newbown rats about 300 days after inoculation. 5) Histology of tumors showed mainly hepatocarcinomas and a few sarcomatous figures. 6) Chromosome numbers of the cultured cells in the 11 cell lines were in the near-diploid range in six of them and in the hypertriploid to hypotetraploid range in the remaining five, when chromosome analysis was done at the time of malignant transformation in vitro.
Published Date
1978-08
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume90
Issue
issue7-8
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
901
End Page
914
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/90/7-8/90_7-8_901/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma