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ID 17274
Eprint ID
17274
FullText URL
Thumnail 90_445.pdf 246 KB
Title Alternative
The levels of human erythrocyte superoxide dismutase activity classified by age and in vitro inhibition by some polluting agents. Part 1 Estimation of red cell superoxide dismutase activity
Author
Ueda, Kazuko
Abstract
The assay of red cell superoxide dismutase (SOD) activity employing the method of nitroblue tetrazolium (NBT) reduction in aerobic xanthine oxidase system was studied. The results were as follows. 1, Determination of unit of SOD activity Assuming the range of NBT reduction is 100 percent, one unit is defined by the amount of SOD required to inhibit NBT reduction by 50 percent. However, the activity defined by this calculating method is lower than the real value. Because the maximal inhibition of NBT reduction in aerobic xanthine oxidase system by crude extract was in the range of 75 to 90 percent in this experiment, and differed from each sample. The activities calculated from the point of 50 percent inhibition and half maximal inhibition showed good correlation (correlation coefficient; 0.92). And to get 50 percent inhibition less samples are needed than to get the maximal inhibition. So, this calculating method could be used in the case of comparison of SOD activities. 2, Recovery of purified SOD 93 percent recovery of purified SOD added to the hemolysate before extraction was obtained. The activities were determined 5 times about the same sample to get the variation coefficient. The value was as low as 2 percent. 3, Interferrence by hemoglobin Methemoglobin formation from acatalasemic hemoglobin by SOD reaction was observed. And maximal inhibition of NBT reduction by hemolysate was low and varies widely. Using hemolysate for sample interferes the assay and the value of SOD activity is unreliable.
Published Date
1978-04-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume90
Issue
issue3-4
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
445
End Page
450
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/90/3-4/90_3-4_445/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma