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ID 16965
Eprint ID
16965
FullText URL
94_1113.pdf 2.28 MB
Title Alternative
Changes in the DNA histogram and the amount of S-100 protein associated with morphological differentiation of cultured glioma cells.
Author
Furuta, Tomohisa
Abstract
A flow cytometric analysis of DNA synthesis and a quantitative investigation of nervous system-specific S-100 protein were performed on cultured rat glioma (C-6) cells in the course of morphological differentiation induced by 1 mM dibutyryl cyclic AMP (dbc-AMP). The flow cytometric analysis revealed that a marked suppression of DNA synthesis was caused by dbc-AMP. The content of S-100 protein in dbc-AMP treated cells began to increase on the fourth day after the treatment with a subsequent increase to approximately 10-fold over the control cells at the stationary phase. These results suggest that S-100 protein is a useful biochemical index for estimating the degree of differentiation of glioma cells.
Keywords
培養グリオーマ細胞
S-100蛋白
micro-complement fixation assay
flow cytometry
DNA ヒストグラム
Published Date
1982-12-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume94
Issue
issue11-12
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
1113
End Page
1125
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/94/11-12/94_11-12_1113/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma