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ID 16340
Eprint ID
16340
FullText URL
95_653.pdf 564 KB
Title Alternative
Measurement of circulating immune complexes using Fc receptors of guinea pig peritoneal exudate cells. Part 1. Establishment of the method for measuring of immune complexes.
Author
Saito, Yoshihito
Abstract
A new method for detecting circulating IgG immune complexes (IC) using guinea pig peritoneal exudate cells (PEC) has been established. Binding of (125)I BSA-anti BSA IgG IC to PEC was inhibited by serum containing IC in a dose dependent fashion. A 100μl aliquot of PEC suspension (2.5X10(6) cells/ml) was preincubated with diluted five fold serum for 60 minutes at 4℃. After washing pretreated PEC 3 times, 100μl of (125)I labelled 3 times antigen excess-IC was added and incubated for 90 minutes at 4℃. At the end of incubation, the suspension was centrifuged at 10,000G for 1 minute, and the radioactivity of the pellet was measured. This method mainly utilizes the reaction between Fc receptors on PEC and the Fc portion of the complexed IgG.
Keywords
immune complex
guinea pig peritoneal exudate cell
Fc receptor
Published Date
1983-08-31
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume95
Issue
issue7-8
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
653
End Page
663
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/95/7-8/95_7-8_653/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma