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ID 16337
Eprint ID
16337
FullText URL
95_637.pdf 237 KB
Title Alternative
Urinary metabolites of organic solvents Report 1: Quantitative determination on urinary metabolites of toluene, xylene and styrene by thin-layer chromatography.
Author
Taguchi, Toyohiro
Abstract
A simple and specific method for the quantitative determination of hippuric acid and methylhippuric acids is described. Urine samples were applied to silica gel plates (silica gel, 60 F254), and developed with chloroform- toluene-methanol-acetic acid (8:2:2:1). The spots of hippuric acid, m- or p-methylhippuric acids, o- methylhippuric acid, mandelic acid and phenylglyoxylic acid were identified by illuminating the silica gel plates with an ultraviolet lamp. The spots of hippuric acid, m- or p-methylhippuric acids and o-methylhippuric acid were scratched off and extracted with pyridine. The color was then developed with acetic anhydride and p-dimethylaminobezaldehyde (DAB) in pyridine at 40℃ for 60min. The absorbance at 458 nm was determined against a blank containing pyridine acetic anhydride and DAB.
Keywords
薄層クロマトグラフィー (Thin-layer chromatography)
トルエン (Toluene)
キシレン (Xylene)
スチレン (Styrene)
生物学的暴露指標 (Biological monitoring)
Published Date
1983-08-31
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume95
Issue
issue7-8
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
637
End Page
642
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/95/7-8/95_7-8_637/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma