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ID 16332
Eprint ID
16332
FullText URL
95_601.pdf 2.61 MB
Title Alternative
Lipid composition of the unstable L-form derived from Pseudomonas aeruginosa IFO 3455.
Author
Naito, Nobuaki
Abstract
The effects of removing the cell wall on the lipid composition of the plasma membrane of Gram-negative Ps. aeruginosa were examined, and compared with the effects observed with Gram-positive bacteria like S. aureus (Hayami et. al, Microbiol. Immunol. 23(6), 1979). The L-form, which was derived from Ps. aeruginosa IFO 3455 by treatment with EDTA-lysozyme, was comprised of 71.5% phospholipids(PL), 5.3% glycolipids(GL) and 23.2% neutral lipids(NL). The phospholipids included phosphatidylethanolamine(PE), cardiolipin(CL), phosphatidylglycerol(PG), and phosphatidylcholine(PC) at the relative concentrations of 53, 27, 12, and 5% , respectively. In comparison with the bacterial form, neutral lipids and CL content was higher in the L-form, but PE was lower. Fatty acid analysis showed that the L-form contained more C(16) saturated fatty acid than the bacterial form. The L-form also contained the C(17cyc') C(19cyc') and C(18-OH) types of fatty acids which were not detected in the bacterial form. These findings are similar to those obtained with S. aureus, indicating that the phospholipids and fatty acids in the plasma membrane play an important role in adaptational changes or compensation for the loss of the cell wall.
Keywords
P. aeruginosa
Spheroplast
L-form
lipid composition
Cardiolipin
Published Date
1983-08-31
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume95
Issue
issue7-8
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
601
End Page
610
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/95/7-8/95_7-8_601/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma