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ID 16021
Eprint ID
16021
FullText URL
Thumnail 99_1257.pdf 544 KB
Title Alternative
Studies on (45)Ca uptake and histamine release by rat peritoneal mast cells Part I Purification and reactivity of rat peritoneal mast cells
Author
Ohtani, Jun
Abstract
Purification of mast cells from the peritoneal cavity of rats and reactivity of the cells to antigen were studies. After peritoneal lavage, the peritoneal cells were washed, the mast cells were isolated by a density gradient centrifugation method. The recovery rate of mast cells was highest when mast cells were separated by BSA density gradient centrifugation after washing the cells on time by low speed centrifugation (50xg). The purity of mast cells obtained by this method was 94.1±1.0%, and the number of mast cells obtained was approximately 1.46×10(6) cells/rat. Mast cells of actively sensitized rats showed a significant increase in (45)Ca uptake and histamine release after stimulation by antigen. (45)Ca uptake by mast cells of passively sensitized rats was affected by BSA medium, and the most suitable results were obtained using mast cells passively sensitized before separation of the cells by BSA. Mast cells showed a significant increase in (45)Ca uptake after stimulation by various stimulatory agents, such as Con. A, Comp. 48/80 and Ca ionophore A 23187. These results indicate that rat peritoneal mast cells isolated by the present method can be used in studies on Ca(2+) influx into the cells.
Keywords
rat peritoneal mast call
purification
reactivity
(45)Ca uptake
histamine release
Published Date
1987-10-31
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume99
Issue
issue9-10
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
1257
End Page
1267
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/99/9-10/99_9-10_1257/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma