このエントリーをはてなブックマークに追加
ID 14236
Eprint ID
14236
FullText URL
Thumnail 104_137.pdf 419 KB
Title Alternative
Estrogen binding capacity in cytosol fraction of human uterine cervix : effect of estrogen and anti-estrogens
Author
Kobayashi, Shunzo
Abstract
The estrogen binding activity in the human uterine cervix was measured, and the effect of natural and synthesized steroids on the activities was determined. To assay the estrogen binding activity, the asmple was incubated with 10nM [(3)H] estradiol-17β at 30℃ for 2 hours. Then dextran-coated charcoal (DCC) was added to a final concentration of 0.5% to separate bound/free (B/F) estradiol. Estrogen binding activity was determined by subtracting the activity found in the heated sample from the corresponding activity in the untreated sample. For this purpose it was found appropriate to heat the sample at 40℃ for 60 minutes. The dissociation constant obtained from the Scatchard plot analysis was : kd=2.0×10(-9)M. A variety of steroids at the same molar concentration (1.0 μM) were added to the sample to determine their effects on the estrogen binding activity. For binding with [(3)H] estradiol-17β, the synthetic estrogens were strongly inhibitory, the anti-estrogen agents were strong-ly to moderately inhibitory. Dannazol, which has been used for the treatment of en-dometriosis, was found to be as effective as androgens. All of those inhibitory effects occur-red in a non-competitive manner.
Keywords
uterine cervix
estrogen receptor inhibition
Published Date
1992
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume104
Issue
issue1-2
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
137
End Page
144
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/104/1-2/104_1-2_137/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma