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ID 14234
Eprint ID
14234
FullText URL
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Title Alternative
Persistent herpes simplex virus type 2 infection in a hamster kidney cell line with a characteristic to grow at a high culture temperature
Author
Kitamura, Tetsuro
Abstract
A subline of the baby hamster kidney cell line (BHK-21), which was adapted to grow at 41℃ and named BHK-21H, was used to examine the effect of pre-treatment of host cells with a high culture temperature on the growth of herpes simplex virus (HSV). BHK-21H cells were infected with HSV type 2 (HSV-2) at 41℃ and maintained for 7 days at the same temperature. When these cells were further cultivated after shift-down to 36℃, persistent infections were established. These infections were characterized by a carrier culture state ; the infected cultures were composed of infected and non-infected cells, and cell destruction and regrowth of cells caused a balanced state of culture. Two to 3 months after the establishment of the persistent infections, virus production in the cultures stopped spontaneously and colonial regrowth of uninfected cells was observed. A subline (BHK-21R) which was cured of the persistent infection was obtained. Cells of this subline showed resistance to reinfection of HSV. Persistent HSV-2 Infection was also established by pre-treatment of BHK-21H cells at 41℃ before virus infection and maintenance of the infected cultures at 36℃. These findings indicate that establishment of the persistent HSV-2 infection is mainly attributable to reduced permissiveness of BHK-21H cells for virus growth caused after heat treatment of the cells.
Keywords
herpes simplex virus
persistent infection
supraoptimal temperature
carrier culture state
permissiveness
Note
原著
Published Date
1991-08
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume103
Issue
issue7-8
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
859
End Page
867
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/103/7-8/103_7-8_859/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
References
1) Darlington RW and Granoff A : Replication-biological aspects ; in The herpesviruses, Kaplan AS ed, Academic Press, New York and London (1973) pp93-132.
2) Roizman B and Sears AE : An inquiry into the mechanisms of herpes simplex virus latency. Ann Rev Microbiol (1987) 41, 543-571.
3) 山田雅夫,新居志郎:単純ヘルペスウイルスの潜伏感染とその機構Ⅰ.生体レベルの研究.ウイルス (1985) 35, 1-10.
4) 山田雅夫,新居志郎:単純ヘルペスウイルスの潜伏感染とその機構Ⅱ.試験管内のウイルス-細胞相互作用.ウイルス (1985) 35, 11-15.
5) Macpherson I and Stoker M : Polyoma transformation of hamster cell clones-an investigation of genetic factors affecting cell competence. Virology (1962) 16, 147-151.
6) Ejercito PM, Kieff ED and Roizman B : Characterization of herpes simplex virus strains differing in their effects on social behaviour of infected cells. J Gen Virol (1968) 2, 357-364.
7) Nii S : The difference in the cytopathic changes in FL cells infected with different strains of herpes simplex virus. Biken J (1961) 4, 215-216.
8) Yamada M, Uno F and Nii S : In vitro cytopathology and pathogenicity to inbred mice shown by five variants of a laboratory strain of type 1 herpes simplex virus. Arch Virol (1986) 90, 183-196.
9) Thimbury MC : Temperature-sensitive mutants of herpes simplex virus type 2. J Gen Virol (1971) 13, 373-376.
10) Wentworth BB and French L : Plaque assay of herpesvirus hominis on human embryonic fibroblasts. Proc Soc Exp Med (1969) 131, 588-592.
11) Nii S : Plaque formation by three variants of the Miyama strain of herpes simplex virus. Biken J (1969) 12, 251-255.
12) Yamada M : Replication of herpes simplex virus in two cell systems derived from rhesus monkeys. Biken J (1983) 26, 35-47.
13) Rapp F : Experimental studies of latency in vitro by herpes simplex viruses. Prog Med Virol (1984) 30, 29-43.
14) Wigdahl BL, Ziegler RJ, Sneve M and Rapp F : Herpes simplex virus latency and reactivation in isolated rat sensory neurons. Virology (1983) 127, 159-167.
15) Hampar B and Copeland ML : Persistent herpes simplex virus infection in vitro with cycles of cell destruction and regrowth. J Bacteriol (1965) 90, 205-212.
16) Nii S : Persistent infection with herpes simplex virus in vitro. Ⅰ. Establishment and characteristics of persistent herpes simplex virus infection in Earle's L cells. Biken J (1969) 12, 45-67.
17) Nii S : Persistent infection with herpes simplex virus in vitro. Ⅱ. Effect of antibody on the course of herpetic persistency in Earle's L cells. Biken J (1970) 13, 339-352.
18) Yura Y, Terashima K, Iga H, Yanagawa T, Yoshida H, Hayashi Y and Sato M : A latent infection of herpes simplex virus type 2 in a human neuroblastoma cell line IMR-32. Arch Virol (1986) 90, 249-260.
19) Yura Y, Terashima K, Iga H, Yanagawa T, Yoshida H, Hayashi Y and Sato M : Macromolecular synthesis at the early stage of herpes simplex virus type 2 (HSV-2) latency in a human neuroblastoma cell line IMR-32 : repression of late viral polypeptide synthesis and accumulation of cellular heat-shock proteins. Arch Virol (1987) 96, 17-28.
20) Zerbini M, Musiani M and Placa ML : Effect of heat shock on Epstein-Barr virus and cytomegalovirus expression. J Gen Virol (1985) 66, 633-636.
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma