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ID 14194
Eprint ID
14194
FullText URL
Thumnail 104_21.pdf 313 KB
Title Alternative
Antitumor effect of interferon-α, γ and/or tumor necrosis factor-α against human colon cancer line (LoVo) transplanted into nude mice
Author
Kataoka, Kazuhiko
Naomoto, Yoshio
Muro, Masahiko
Kojima, Kazushi
Horiki, Sadayuki
Hizuta, Akio
Tanaka, Noriaki
Orita, Kunzo
Abstract
We investigated the in vivo antitumor effect of recombinant human interferon-α,γ (IFN-α,γ) and/or recombinant human tumor necrosis factor-α (TNF-α) against human colon cancer cells (LoVo) transplanted into nude mice. LoVo cells were very sensitive to IFN-γ and slightly sensitive to IFN-α and resistant to TNF-α in vitro by dye uptake method. Intravenous administration of IFN-γ significantly inhibited tumor growth transplanted subcutaneously into nude mice, but IFN-α and/or TNF-α did not show any antitumor effect. Labeling index on stanining with bromodeoxyuridune and mitotic index of LoVo cells treated with IFN-α,γ or TNF-α differed slightly from those of the control group. We previously reported that the synergistic antitumor effects on the three cell lines could be examined by the combined use of IFN-α and TNF-α and the mechanism of the synergism was arrested in the S phase of cell cycle of target cells. However in the cese of LoVo cells, the combined use of IFN-α and TNF-α was not effective and did not arrest the cells in tha S phase of cell cycle. Therefore, arrest in the S phase was suspected to be responsible from the synergistic antitumor activity of IFN-α and TNF-α on the sensitive targets.
Keywords
nude mouse
human colon cancer
interferon
tumor necrosis factor
cell cycle
Published Date
1992
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume104
Issue
issue1-2
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
21
End Page
27
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/104/1-2/104_1-2_21/_article/-char/ja/
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma