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ID 14072
Eprint ID
14072
FullText URL
106_483.pdf 2.52 MB
Title Alternative
Expression of transferrin receptor mRNA in humana erythroblasts : A method for separating erythroblasts and measuring transferrin receptor m RMA
Author
Itami, Shigeto
Abstract
A method for separating erythroblasts and measuring transferrin receptor (TfR) mRNA was developed to investigate the mechanism of TfR expression. Using this method, the relationship between TfR mRNA and the average number of stainable iron granules was examined. Erythroblasts were separated using immunomagnetic beads and mouse antiglyco-phorin A monoclonal antibody with a purity of 89.5±4.5% and a yield of 19.9±6.7%. Northern blot analysis was performed with these separated cells and the following results were obtained. A patient with iron deficiency anemia presented a higher TfR mRNA level and a lower average number of stainable iron granules than healthy volunteers. However, another patient with iron deficiency anemia and two patients with myelodyspastic syndrome demonstrated almost the same TfR mRNA levels as healthy volunteers . TfR mRNA appeared to be regulated by cellular iron, but other factors such as hemoglobim may participate in this regulation. This newly developed method will be helpful in investigating the regulation of TfR mRNA and in clarifying the mechanism of iron metabolism in hematological disorders.
Keywords
transferrin receptor mRNA
Northern blot analysis
erytroblasts
cell separation
immunomagnetic beads
Note
原著
Published Date
1994
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume106
Issue
issue5-6
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
483
End Page
492
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/106/5-6/106_5-6_483/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma