ID 13845
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Author
Kunihiko Seno
Kazuki Matsumura
Oshima, Mitsuko
Motomizu, Shoji
Abstract
1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC·HCl) is a very useful agent to form amide bonds (peptide bonds) in an aqueous medium. A simple and fast detection system was developed using the reaction with pyridine and ethylenediamine in acidic aqueous solution and spectrophotometric flow injection analysis. The absorbances were measured at 400 nm and the reaction was accelerated at 40˚C. The calibration graph showed good linearity from 0 to 10% of EDC·HCl solutions: the regression equation was y = 3.15 × 104x (y, peak area; x, % concentration of EDC·HCl). The RSD was under 1.0%. Sample throughput was 15 h–1. This method was applied to monitoring the EDC·HCl concentration that remained after the anhydration of phthalic acid in water, esterification of acetic acid in methanol or dehydration condensation of malonic acid and ethylenediamine in water.
Published Date
2008-04-10
Publication Title
Analytical Sciences
Volume
volume24
Issue
issue4
Publisher
日本分析化学会
Start Page
505
End Page
508
ISSN
0910-6340
NCID
AA10500785
Content Type
Journal Article
language
英語
OAI-PMH Set
岡山大学
Copyright Holders
日本分析化学会
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publisher
DOI