109_151.pdf 1.82 MB
Physical development of tissue-reacted cationic colloid with protein silver and gold chloride
Su, Wei Dong
Hong, Luo Jia
Piao, Da Xun
Rat brain sections were treated with fine granular cationic iron colloid, which was prepared by boilling ferric chloride with hydrazine hydrate and cacodylic acid (Murakami et al., 1986). The sections were then treated with ferrocyanide and HCl for Prussian blue reaction or with protein silver and gold chloride for physical development. Physical development had an advantage over Prussian blue reaction in that it produces clear and highly contrasted images under a light microscope. Thus, the perineuronal sulfated proteoglycans and the fine processes of the nerve cells were clearly demonstrated by physical development.
Cationic iron colloid
Prussian blue reaction
Bodian's silver impregnation
|Publication Title Alternative||
Journal of Okayama Medical Association
Okayama Medical Association
1) Seno S, Akita T, Ono T and Tsuji T : Fine-granulara cationic iron colloid. Its preparation, physiochemical characteristics and histochemical use for the detection of ionized anionic groups. Histochemistry (1985) 82, 307-312.
2) Murakami T, Taguchi T, Ohtsuka A and Sano K, Kaneshige T, Owen RL and Jones AL : A modified method of fine-granular cationic iron colloid preparation : It use in light and electron microscopic detection of anionic sites in the rat kidney glomerulus and certain other tissues. Arch Histol Jpn (1986) 49, 13-23.
3) Murakami T, Taguchi T, Ohtsuka A and Kikuta A : Neurons with strongly negative-charged surface-coats in adult rat brain as detected by staining with cationic iron colloid. Arch Histol Jpn (1993) 56, 13-21.
4) Murakami T, Ohtsuka A, Taguchi T : Neurons with intensely negatively charged extracellular matrix in the visual cortex. Arch Histol Cytol (1994) 57, 509-522.
5) Murakami T, Ohtsuka A, Taguchi T and Piao DX : Perineuronal sulfated proteoglycans and dark neurons in the brain and spinal cord : A histochemical electron microscopic study of newborn and adult mice. Arch Histol Cytol (1995) 58, 557-565.
6) Ueda H, Fujimori O and Abe M : Histochemical analysis of acidic glycoconjugates in the endothelium lining the splenic blood vessels in the rat. Arch Histol Cytol (1996) 59, 389-397.
7) Toda K, Nishida K, Inoue H, Ohtuska A and Murakami T : Strongly anionic sites in peripheral axons of the rat scatic nerve : Light and electron microscopic detection using cationic iron colloid. Arch Histol Cytol (1995) 58, 485-492.
8) Murakami T, Ohtsuka A and Ono K : Neurons with perineuronal sulfated proteoglycans in the mouse brain and spinal cord : Their distribution and their reactions to lectin Vicai villosa agglutinin and Golgi's silver nitrate. Arch Histol Cytol (1996) 59, 219-231.
9) Bodian D : A new method for staining nerve fibers and nerve engings in mounted paraffin sections. Anat Rec (1936) 65, 89-97.
10) Bodian D : The staining of paraffin of nervous tissues with activated protargol. The role of fixatives Anat Rec (1937) 66, 153-162.
11) Ohtsuka A and Murakami T : Fine anionic iron colloid and its use in light and electron microscopic detection of cationic sites in the rat kidney. Arch Histol Jpn (1986) 49, 543-552.
|Eprints Journal Name||