ID | 13634 |
Eprint ID | 13634
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FullText URL | |
Title Alternative | Establishment and characterization of cell line from ferric nitrilotriacetate (Fe-NTA)-induced rat renal cell carcinoma
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Author |
Kakehashi, Chikako
Mori, Masaharu
Kawabata, Teruyuki
Okada, Shigeru
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Abstract | The characteristics of ferric nitrilotriacetate (Fe-NTA)-induced rat renal cell carcinoma by intraperitoneal administration and the effects of hormones on the tumor have been studied in vivo. However, to our knowledge there have been no reports on the characteristics of the tumor cells. In this study, we established a tumor cell line from Fe-NTA induced rat renal tumour and investigated tumor cell characteristics. The tumor cell line RKFe was established from Fe-NTA induced rat renal tumor by collagenase digestion. RKFe showed epithelial morphology in culture, and had microvilli and basement membranes under electron microscopy. RKFe cells acquired tolerance to cytotoxicity of Fe-NTA. They also grew in soft agar culture and formed tumors by the inoculation of cells into nude mice. Histologically, transplanted tumors showed poorly defferentiated adenocarcinoma with several tubular structures. A subclone of RKFe, named RKFe-C6, showed metastasis to lungs. The RKFe cell line may be useful for studying the characteristics of Fe-NTA induced rat tumor cells.
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Keywords | Fe-NTA
腎癌
培養細胞株の樹立
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Note | 原著
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Published Date | 2001-04-28
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Publication Title |
岡山医学会雑誌
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Publication Title Alternative | Journal of Okayama Medical Association
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Volume | volume113
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Issue | issue1
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Publisher | 岡山医学会
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Publisher Alternative | Okayama Medical Association
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Start Page | 33
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End Page | 39
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ISSN | 0030-1558
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NCID | AN00032489
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Content Type |
Journal Article
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Official Url | https://www.jstage.jst.go.jp/article/joma1947/113/1/113_1_33/_article/-char/ja/
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Related Url | http://www.okayama-u.ac.jp/user/oma/index.html
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language |
Japanese
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Copyright Holders | 岡山医学会
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File Version | publisher
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Refereed |
True
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Eprints Journal Name | joma
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