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ID 13570
Eprint ID
13570
FullText URL
114_133.pdf 2.09 MB
Title Alternative
A study on thr effect of Heliobacter pylori to the gastric mucosal defensive mechanism
Author
Yoshinaga, Fumiya
Abstract
UDP-galactosyltransferase (UDP-Gal-T) is a key enzyme in the synthesis of mucus glycoproteins, which play an important role in gastric mucosal defensive mechanisms. Helicobacter pylori (HP) induces gastritis and peptic ulceers but mechanisms of HP-induced mucosal injury sre not fully understood. To elucidate the mechanism whereby (HP) infection induces gastric mucosal injury, we investigated the effects HP on the gastric mucosal defensive mechanism by measuring UDP-Gal T activies in HGC-27, a mucin-producing gastric caneer cell line, treated with various HP-relatd materials (sonicated HP, HP culture supernatants, live HP). HP-related materials induced no significant chages in UDP-Gal T activity, but the enzyme increased when we generated a low concentration of ammonia by adding sonicated HP. and urea simultaneousy. In contrast, UDP-Gal-T activity did not increase when we added live HP instead of sonicated HP. Next, we investigated the effects of HP on ethanol-induced injury in the HGC-27 cells. Pretreatment with ammonia of a low concetration significantly protected cells from ethanol-induced injury. In contrast, pretreatment with live HP and urea generated ammonia of the similar concentration but did not protect cells from the injoy. Our findings that HP has an inhibitory effect against adaptive cytoprotection induced by ammonia by inhibiting the induction of UDP-Gal T.
Keywords
Helicobacter pylori
UDP-galactosyltansferase
ammonia
ethanol
adaptive cytoprotection
Note
原著論文
Published Date
2002-09-30
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume114
Issue
issue2
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
133
End Page
138
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/114/2/114_2_133/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/index.html
language
日本語
Copyright Holders
岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma