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ID 13475
Eprint ID
13475
FullText URL
Thumnail 117_219.pdf 5.37 MB
Title Alternative
Characterization and usefulness of a monoclonal antibody against human herpesvirus-7 DNA polymerase processivity factor
Author
Abstract
A monoclonal antibody (MAb), IK17, was developed and found useful for the analysis of human herpesvirus-7 (HHV7) genome replication. In Western blot analysis, the MAb IK17 specifically detected an HHV7 DNA polymerase subunit,U27 protein,expressed in both Escherichia coli (E.Coli)and HHV7-infected cells.Analysis by the same method of truncated U27 proteins expressed in E. Coli demonstrated that the MAb IK17 recognizes an epitope between amino acid 1 and 133 of the U27 protein.The indirect immunofluorescence (IF)method with MAb IK17 detected an unexpected reduction of U27 protein in the infected cells that were treated with a viral DNA polymerase inhibitor,phosphonoacetic acid (PAA),although no reduction of U27 mRNA was observed in the cells by Northern blot analysis. The data suggest the possible usefulness of the MAb IK17 for obtaining clues to characterize an unknown feature of the U27 protein. Immunoprecipitation experiments with the MAb IK17 enabled to detect at least four U27 protein-associated phosphorylated polypeptides in addition to the U27 protein itself.The result indicates the usefulness of the MAb IK17 for qualitative and quantitative analysis of the U27 proteinassociated viral and cellular factors. These analyses contribute to elucidation of the HHV7 genome replication system.
Keywords
ヒトヘルペスウイルス7型 (Human Herpesvirus-7)
DNA polymerase processivity factor
単クローン抗体 (monoclonal antibody)
Note
原著
Published Date
2006-01-04
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume117
Issue
issue3
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
219
End Page
224
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/117/3/117_3_219/_article/-char/ja/
Related Url
http://www.okayama-u.ac.jp/user/oma/
language
日本語
Copyright Holders
Copyright© 岡山医学会
File Version
publisher
Refereed
True
Eprints Journal Name
joma