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ID 13423
Eprint ID
13423
FullText URL
Thumnail 117_41.pdf 6.36 MB
Author
Sakaguchi, Yoshihiko
Abstract
Clostridium botulinum types C and D toxin production is governed by the specific bacteriophages. A type C phage,c-st,was isolated from a toxigenic strain of C-Stockholm,and its whole genome sequence was determined.The genome was a linear double-stranded 185,681bp DNA with a G+C content of 26.2%, and identical 403-bp sequences were found at each end of the genome. From the DNA research, 198 potential protein-coding regions (ORFs)were identified. The genes for type C progenitor toxin and C3 exoenzyme were identified, but no type D toxin gene was found.A number of genes were homologous to those of a Bacillus subtilis bacteriophage,SP D. On Southern blot analysis between type C and D cell DNAs and the specific probes for H and L chains of C and D neurotoxins, it became clear that type C and D strains posses only one type of toxin gene.From these findings, it is concluded that cross-neutralization observed between toxins (types C and D)and their antiserums can be caused by the common-antigens in C and D toxins.
Keywords
ボツリヌス菌 (Clostridium botulinum)
ファージ (phage)
神経毒素遺伝子 (neurotoxin gene)
Note
原著
Published Date
2005-05-20
Publication Title
岡山医学会雑誌
Publication Title Alternative
Journal of Okayama Medical Association
Volume
volume117
Issue
issue1
Publisher
岡山医学会
Publisher Alternative
Okayama Medical Association
Start Page
41
End Page
46
ISSN
0030-1558
NCID
AN00032489
Content Type
Journal Article
Official Url
https://www.jstage.jst.go.jp/article/joma1947/117/1/117_1_41/_article/-char/ja/
References
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language
日本語
Copyright Holders
Copyright© 岡山医学会
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publisher
Refereed
True
Eprints Journal Name
joma